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Throughout the tropical and subtropical climates, the genus Citrus can be found. The current study was conducted to extract the <i>Citrus sinensis</i> peel oil and evaluate its antibacterial, antifungal and antiparasitic potential. Petroleum ether was used to extract the <i>C. sinensis</i> peel oil through a Soxhlet apparatus. The antimicrobial and antifungal potential was determined via agar well diffusion method and minimum inhibitory concentrations (MIC) were calculated (test bacterial strains: <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Streptococcus agalactiae</i>; test fungal strains: <i>Aspergillus flavus</i>, <i>Aspergillus niger</i>, <i>Altrnaria alternata</i>). Antiparasitic activity against <i>Leishmania</i><i>tropica</i> was determined following standard protocol using amphotericin-B as positive and Dimethyl Sulfoxide (DMSO) as a negative control and the percentage inhibition was calculated. The oil extracted was brownish yellow with a tangy smell, water-insoluble, density (0.778 g/cm³) and specific gravity (0.843 g/cm). In antibacterial activity, the diameter of the zone of inhibition was maximum against <i>E. coli</i> (14 mm) and minimum for <i>S. agalactiae</i> (10 mm). While in antifungal activity diameter of the zone of inhibition was maximum against <i>A. flavus</i> (12.5 mm) and minimum for <i>A. alternata</i> (8.6 mm). <i>S. agalactiae</i> exhibited the minimum MIC value (6 mg/mL) and in fungal strains <i>A. alternata</i> exhibited the minimum value (2 mm). <i>Citrus sinensis</i> peel oil displayed antileishmanial efficiency of 60% at 50 μg/mL concentration after 48 h of incubation. <i>C. sinensis</i> peel oil demonstrated antimicrobial capabilities, implying that it could be used as a natural preservative in food or as an effective treatment against a variety of pathogenic organisms. Industries should extract oil from the waste of citrus fruits which will be beneficial from an economic point of view.