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Alternaria brown spot disease is caused by the <i>Alternaria alternata</i> tangerine pathotype, which relies on ACT-toxin for infection. At present, all identified ACT-toxin biosynthesis-related genes are multi-copy genes. In this study, we summarized the advances in important host-specific toxins (HSTs), and listed key genes required for the pathogenicity of the <i>A. alternata</i> tangerine pathotype. Toxin virulence test results revealed that different citrus species displayed distinctly different tolerances to ACT-toxin. The extraction method of ACT-toxin crude extract was described in schematic form to make the method easier to understand. In addition, target gene disruption of two copies of <i>ACTT5</i> (<i>∆∆ACTT5</i>) displayed significantly reduced virulence, indicating that <i>ACTT5</i> is essential for the pathogenicity of the <i>A. alternata</i> tangerine pathotype.