Find in Library
Search millions of books, articles, and more
Indexed Open Access Databases
Antagonism of selected Arecaceae endophytic bacteria against Curvularia sp. leaf spot pathogen isolated from coconut kopyor
oleh: Deden Dewantara Eris, Agus Purwantara, Abdul Munif, Bonny Poernomo Wahyu Soekarno
Format: | Article |
---|---|
Diterbitkan: | indonesian research institute for biotechnology and bioindustry 2018-10-01 |
Deskripsi
Coconut kopyor is one of the most important commodities. One of the problems in coconut kopyor cultivation is grey leaf spot disease caused by Curvularia sp. Using endophytic bacteria is one of the control technique that is environmentally friendly. A total of 40 selected endophytic Arecaceaebacteria isolated from coconut kopyor, palm oil, aren, nibung and pejibaye were tested for their inhibitory ability to Curvularia sp.through antibiotic and volatile organic compound (VOC)test.The antibiotic test showed that thirty three endophytic bacteria isolates have inhibitory capacity againstCurvularia sp. in a range of inhibition from 4.4% to 86.6%. Isolates with the highest inhibition were EAKSS 502, EAKSS 520 and EAKSS 507. VOC test showed that EAPJN 216, EAKSS 532, EAAPN 225, EAAPN 506, EAAPN 507 and EAAPN 557 were produced VOC that suppressed the growth of Curvulariasp fungal colonies in a range from 92.27% to 97.21%. Based on the best combination of antibiotic and production of volatile organic compound test, there were four potential isolates to inhibit the growth of Curvulariasp. in vitro i.e. EAKSS 502, EAKSS 507, EAKPN 201 and EAPJN 216. Those isolates were molecularly identified as Serratia marcescensstrain PIGB81, Burkholderiasp. DOP Ma316, S. marcescensstrain RY21 and S. marcescensstrain LB21.The four isolates were isolated from different plants such oil palm, coconut kopyor and pejibaye.