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In this study, we investigated the role of liver X receptor (LXR) in regulating cholesterol concentration in goose primary hepatocytes. Intracellular and extracellular cholesterol concentration, mRNA expression levels of genes related to phosphatidylinositol 3- kinase (PI3K) pathway, sterol regulatory element- binding protein 2 (SREBP-2), and 3- hydroxy-3-methylglutaryl-CoA reductase (HMGR) were measured in primary goose hepatocytes. We found that the intracellular cholesterol concentration showed an uptrend manner when the TO901317 concentration was under 1 μmol/L; compared with 1 μmol/L TO901317, 10 μmol/L TO901317 had an inhibiting effect (P&lt;0.05). The regulation mode of <em>SREBP-2</em> and <em>HMGR</em> gene expression is similar with that of intracellular cholesterol concentration. These data suggested that LXR activation may stimulate the cholesterol biosynthesis by activating expression of <em>SREBP-2</em> and <em>HMGR</em>. Interestingly, the mRNA levels of genes related with PI3K pathway increased in the presence of TO901317, which suggested that LXR activation could promote PI3K signalling activity.