Novel ecto-tagged integrins reveal their trafficking in live cells
oleh: Clotilde Huet-Calderwood, Felix Rivera-Molina, Daniel V. Iwamoto, Emil B. Kromann, Derek Toomre, David A. Calderwood
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-09-01 |
Deskripsi
Integrins are cell-surface adhesion receptors that are modulated by endo-exocytic trafficking, but existing tools to study this process can interfere with function. Here the authors develop β1 integrins carrying traceable tags in the extracellular domain; a pH-sensitive pHlourin tag or a HaloTag to facilitate dye attachment.