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Chi-B partial sequences of Sorghum halepense and its relatives were amplified with primer D16/D18 derived from Chi-B of the Sorghum genus. The results showed that the length varied from 567 bp to 607 bp, while phylogenetic relationship analysis by "DNASTAR" software showed that all materials were separated into three sections. The first section included S. halepense, S. almum and S. silk with a similarity of 96.9~100%, the S. bicolor, S. propinquum and S. arumdinaceum species were in sections of two with 93.0~95.3% similarity, the genetic distance between S. nitidum and the others was longer than in the case of the others, with a similarity of 69.0~71.0%. With accession-special primer D51/D55, S. halepense and S. almum could be identified by PCR.