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Often ignored musculoskeletal pain among adolescent school students can result in the development of musculoskeletal disorders in adulthood. To determine the prevalence of musculoskeletal pain and its risk factors, a cross-sectional study was conducted on 1600 students (855 males and 745 females) registered in 10 co-educational government schools of Delhi. Demographic details along with Nordic Musculoskeletal, Depression, Anxiety Stress Scale-21, Youth physical activity and modified Sedentary Behaviour Questionnaires (duration of using smartphones and watching television) were administered. The prevalence of musculoskeletal pain was 63% (55-70) at 95% CI. The female gender (p=0.008), moderate to vigorous level physical activity more or less than 60-90 minutes per day (p=0.017), smartphone use ≥ 5 days a week and ≥ 2 hours per day (p=0.02) were the risk factors for developing musculoskeletal pain. The stress (p=0.037), anxiety (p=0.032), and depression (p=0.056) were associated with musculoskeletal pain in Chi-square (Χ2) analysis.