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This research reported the in vitro pathogenicity of <i>Verticillium lecanii</i> strains, L2 and L5, and <i>Beauveria bassiana</i> strains, B76 and B252, against <i>Megoura japonica</i> using leaf-dip method. The virulence potential of these four entomopathogenic fungal strains of <i>V. lecanii</i> and of <i>B. bassiana</i> were compared between fungi conidia (concentrations 1 × 10⁶, 1 × 10⁷, and 1 × 10⁸ conidia mL⁻¹) and culture filtrate. Moreover, binary combination of four different fungal strains (L2 + B76, B76 + L5, L2 + B252, and L2 + B76 + B252 + L5) were evaluated against <i>M. japonica</i> under control condition. Aphid mortality was recorded after two, four, six, and eight days of post-treatment. In the conidial concentration bioassay, strain B76 showed maximal mortality (85.3%) against bean aphid, and strain L5 showed the lowest effect (60.0%) at the highest concentration (1 × 10⁸ conidia mL⁻¹) at eight days post-treatment. Whereas, binary combinations of B76 and L2 strains showed the highest effect against <i>M. japonica</i> (90.5%) than other combinations. Moreover, in comparison with the effect of filtrate and conidia bioassay, 91.4% and 84.1% were achieved in strain B76, and the lowest effect (63.8% and 55.1%) was recorded in strain L5.