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A cell-on-electrode-on paper device (CEP-D) was developed to allow for simultaneous cell maintenance and electrochemical measurements. Precise quantification of neurotransmitters (here dopamine, DA) secreted by exocytosis from a population of PC-12 cells maintained in filter paper coated with PEDOT-PSS is demonstrated. A specific calibration protocol, based on the self-normalization of voltammetric data, is presented. Robust, reproducible, and low-cost measurements were achieved, confirming the release of DA after stimulation. Importantly, the impact of different drugs on the exocytotic machinery was tested, and the data obtained is consistent with previously reported experiments at the single cell level. Overall, and beyond the biological results, this study shows that simple, benchtop techniques can be used to obtain precious biochemical data from cells cultured on paper if the analytical protocol is carefully designed to account for the challenges of biomeasurements. This new approach paves the way for the design of smart ‘organs-on-paper’ that are affordable, precise and easy to use and produce, thus addressing several challenges of the current technology.