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A virulent strain of Edwardsiella tarda isolated from an outbreak in an organized Clarias batrachus hatchery was used to study the infectivity pattern and pathology in Anabas testudineus. The 50% lethal dose for A. testudineus injected intraperitoneally with E. tarda was 107.8 cells. The organism produced acute disease pattern with characteristic histopathological lesions viz., hepatic necrosis and intravascular thrombus, myocardiopathy and enteritis along with mild necrosis in the spleen and kidney. E. tarda specific antigen was detected in the liver, kidney and spleen of dead fish through dot-ELISA.