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Premise of the study: We developed microsatellite markers for the endangered plant <i>Primula boveana</i>, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. Methods and Results: DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in <i>P. boveana</i>. Eleven of these primers successfully cross-amplified to related taxa. Conclusions: The markers reported herein will be useful to characterize the genetic diversity of the endangered <i>P. boveana</i> and to evaluate its mating system, and have the potential to be useful for similar studies in close relatives.