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Introduction and Aims This study aimed to determine the relationship between job satisfaction and job stress and its associated factors associated in EMS personnel of Mazandaran University of Medical Sciences. Materials and Methods Cross-sectional study was that a total of 150 emergency personnel affiliated to Mazandaran University of Medical Sciences, 150 questionnaires were completed and returned. Data collection questionnaires were researcher made job satisfaction and HSE job stress. Content validity was confirmed by expert consultant and the reliability coefficient were calculated by the test-retest method in %10 of cases (N = 15) at 2-wk intervals. Total cronbach&rsquo;s alpha coefficient was 0.85 and for dimensions it was 0.8 to 0.91. In data analysis SPSS v.21 software and descriptive statistics (mean, standard deviation and correlation coefficient) and inferential statistics (ANOVA, Fisher&rsquo;s exact test and Pearson&rsquo;s correlation coefficient) were used in significant level of (P < 0.05). Results According to the results the most level of job satisfaction was related to workplace conditions and the lowest was in the relationship with one&rsquo;s life and job security. Analysis of variance showed significant differences between job satisfaction and job stress and its related factors, there is meaningful relationship (P < 0.05). Also there was a significant relationship between job satisfaction among emergency personnel and some individual characteristics such as age and working experience (P < 0.05). Conclusion Job satisfaction of EMS personnel is a vital factor in health and immune system that causes to improvement of performance, quality of healthcare and patient satisfaction and reduction of costs. * Corresponding Author: Babol University of Medical Sciences, Ayatollah Ruhani Hospital. E-mail: Hamedhamedya@yahoo.com