Generation of an urine-derived induced pluripotent stem cell line WMUi017-A from a X-linked Renpenning syndrome (X-RSY) patient with the hemizygous PQBP1 gene mutation p.P609A (c.1825C>G)
oleh: Xiaoling Guo, Xufei Zhang, Linlin Wang, Lingyun He, Yinjuan Ding, Huihui Chen, Dexuan Wang, Xing Rong, Xian Shen, Jian Lin, Maoping Chu
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-03-01 |
Deskripsi
The mutations of polyglutamine binding protein 1 gene (PQBP1) can lead to the rare inherited X-linked Renpenning syndrome (X-RSY). Here, an induced pluripotent stem cell (iPSC) line WMUi017-A was generated through reprogramming the urine cells of a 5-year-old male X-RSY patient with the hemizygous PQBP1 gene mutation p.P609A (c.1825C>G) using the commercial Sendai virus reprogramming system. The established iPSCs can stably express pluripotent stem cell markers OCT4 and NANOG, and can be induced into three germ layers and maintain a normal karyotype (46, XY) in vitro.