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ObjectiveEstablishment and optimization of arachidonic acid-induced diffuse cerebral thrombosis model in rats.MethodsArachidonic acid was injected into the internal carotid artery to establish a diffuse cerebral thrombosis model in rats, while the sham operation group was set as the control. The model was evaluated by detecting the exudate content of Evans blue (EB) in brain tissue and the pathological damage degree of brain tissue by HE staining.ResultsCompared with the sham operation group, diffuse EB exudation was observed in the injured brain tissue of the model group (P < 0.01), and the exudation was relatively uniform. The HE staining of brain tissue on the side of the injury showed ischemic pathological damage with disorganized cell arrangement, wrinkled nuclei, enlarged cell gaps, increased vacuolated cells, and red striped tubular shape of each individual microvessel. Conclusion Arachidonic acid can successfully induce diffuse cerebral thrombosis model, and the optimized model has better stability and lower mortality.