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Arthrobotrys oligospora is a potential candidate of biocontrol agents against plant and animal parasitic nematodes. In this study, the complete mitochondrial genome of A.oligospora was sequenced. This mitogenome is a circular molecule of 160,613 bp in length. Gene annotation showed that 44 putative protein-coding genes and 24 tRNAs, all located on the same strand. The evolutionary relationships between A. oligospora and other representative ascomycetes were revealed based on sequences at the 14 concatenated mitochondrial protein-coding genes.