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Twenty dairy cows were used in an experiment at which the influence of silages with different content of acids on the rumen fermentation was studied. Animals were divided into experimental and control group, each of them about 10 individuals. Feeding ratio consisted of maize silage (26 kg), alfalfa silage (13 kg), meadow hay (1 kg) and corn mixture (8.5 kg). The experimental group was fed an inoculated maize silage, with higher contents of fermentation acids. Intake of total acids per 1 kg live weigh was higher in the experimental group than control group (1.98 g/kg; 1.48 g/kg live weight, respectively). The rumen fluid was taken per orally 2-3 hours after feeding. Mean value of pH of rumen fluid was significantly (PThe most significant increase (P0.05) lower in experimental group. A protozoa (infusoria) content was significantly (P<0.01) lower in the experimental group (175.76 ± 12.54 thousand/ml) as compared with the control group (288.1 ± 13.73 thousand/ml).