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Abstract In the spirit of getting back to nature and using science to increase crop productivity without posing any threat to the environment, researchers are paying attention to making natural products alternative sources of nutrients for plants at affordable prices. On top of this, chitosan and cyanobacteria have become popular in agriculture as metabolic enhancers, biofertilizers, and antimicrobial properties. Cyanobacteria are known to possess biostimulating properties while chitosan is well known for its inherent biological properties. With the aim of minimizing the application of nitrogen, this experiment was conducted for the first time to check if the application of chitosan, microalgae, or both with 50% nitrogen can balance the nutrient requirement for different physiological and biochemical development as effectively as a 100% nitrogen dose. The data were recorded only for the early vegetative stages, as the seeds were non‐vernalized. The basic parameters recorded were hexose content, chlorophyll a, chlorophyll b, total phenol content, and relative water content (RWC). In most of the parameters, comparable results were found between the control (with a 100% nitrogen recommended dose) and other treatments (where either microalga, chitosan, or both were added), whereas it was clearly shown that 50% of recommended nitrogen doses reduce the hexose, chlorophyll, and RWCs. Thus, the treatments were effective in supplementing the developmental requirements. Therefore, the combined use of chitosan and cyanobacteria on crops significantly lowers nitrogen fertilization, increases photosynthesis, enhances resistance to water stress, and enhances antioxidant activity in modern agriculture.