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<i>Besnoitia besnoiti</i> tachyzoites infect and develop in bovine endothelial cells in vivo and trigger the release of neutrophil extracellular traps (NETs) from bovine polymorphonuclear neutrophils (PMN). The purpose of this study was to analyze if pure <i>B. besnoiti</i> tachyzoite-triggered NETs would damage endothelial host cells and subsequently influence intracellular development and proliferation of <i>B. besnoiti</i> tachyzoites in primary bovine endothelial cells. For comparison purposes, isolated A23187-induced NETs were also used. Thus, we here evaluated endothelial host cell damage triggered by histone 2A (H2A) and <i>B. besnoiti</i> tachyzoite-induced NET preparations and furthermore estimated the effects of PMN floating over <i>B. besnoiti</i>-infected endothelium under physiological flow conditions on endothelial host cell viability. Overall, all treatments (H2A, <i>B. besnoiti-</i>triggered NETs and floating PMN) induced endothelial cell death of <i>B. besnoiti</i>-infected host cells. However, though host cell damage led to significantly altered intracellular parasite development with respect to parasitophorous vacuole diameter and numbers, the total proliferation of the parasite over time was not significantly affected by these treatments thereby denying any direct effect of NETs on intracellular <i>B. besnoiti</i> replication.