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Detection of copper (II) ions (Cu²⁺) in water is important for preventing them from entering the human body to preserve human health. Here, a highly sensitive and selective fluorescence probe that uses mercaptopropionic acid (MPA)-capped InP/ZnS quantum dots (MPA-InP/ZnS QDs) was proposed for the detection of trace amounts of Cu²⁺ in water. The fluorescence of MPA-InP/ZnS QDs can be quenched significantly in the presence of Cu²⁺, and the fluorescence intensity shows excellent linearity when the concentration of Cu²⁺ varies from 0–1000 nM; this probe also exhibits an extremely low limit of detection of 0.22 nM. Furthermore, a possible fluorescence-quenching mechanism was proposed. The MPA-InP/ZnS QDs probes were further applied to the detection of trace Cu²⁺ in real water samples and drink samples, showing good feasibility.