Rescue of conformational dynamics in enzyme catalysis by directed evolution
oleh: Renee Otten, Lin Liu, Lillian R. Kenner, Michael W. Clarkson, David Mavor, Dan S. Tawfik, Dorothee Kern, James S. Fraser
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-04-01 |
Deskripsi
A key challenge in the field of protein design and evolution is to understand the mechanisms by which directed evolution is improving enzymes. Here the authors combine different biophysical methods and give mechanistic insights into how directed evolution increases the catalytic efficiency of human peptidyl-prolyl cis/trans isomerase CypA.