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<div>Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containing</div><div>therapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer,</div><div>antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known as</div><div>duku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties is</div><div>nearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences of</div><div>the ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogenetic</div><div>relationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status of</div><div>duku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNA</div><div>sequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotide</div><div>sequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from</div><div>18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite for</div><div>Sequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragments</div><div>ranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku,</div><div>kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. It</div><div>was determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku,</div><div>kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with the</div><div>similarity index value of 0.996.</div><div><br /></div><div>Keywords : Phylogenetic relationship, ITS region, L. domesticum, duku, kokosan, langsat</div>