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Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
oleh: Li Wang, Hilal Betul Kaya, Hilal Betul Kaya, Ning Zhang, Rhitu Rai, Rhitu Rai, Matthew R. Willmann, Sara C. D. Carpenter, Andrew C. Read, Federico Martin, Zhangjun Fei, Jan E. Leach, Gregory B. Martin, Gregory B. Martin, Adam J. Bogdanove
Format: | Article |
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Diterbitkan: | Frontiers Media S.A. 2021-03-01 |
Deskripsi
Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in Nicotiana benthamiana, rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.