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Iron oxide nanoparticles are produced using simple auto combustion methods with honey as a metal-stabilizing and -reducing agent. Herein, α-Fe₂O₃ nanoparticles are produced using an iron nitrate precursor. These prepared samples are analyzed by an X-ray diffractometer (XRD), FTIR spectroscopy, UV-DRS, and a field-emission scanning electron microscope (FESEM) combined with energy-dispersive spectroscopy and a vibrating sample magnetometer (VSM). The XRD results confirm a rhombohedral structure with an R3<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mover><mi mathvariant="normal">c</mi><mo>¯</mo></mover></mrow></semantics></math></inline-formula> space group single-phase formation of α-Fe₂O₃ in all samples. FESEM images reveal the different morphologies for the entire three samples. TEM analysis exhibits spherical shapes and their distribution on the surfaces. XPS spectroscopy confirms the Fe-2p and O-1s state and their valency. The VSM study shows strong ferromagnetic behavior. The prepared α-Fe₂O₃ nanoparticles exhibit exceptional charge carriers and radical production. The prepared sample retains excellent photocatalytic, antifungal and antibacterial activity.