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The RNA-Binding Landscape of HAX1 Protein Indicates Its Involvement in Translation and Ribosome Assembly
oleh: Anna Balcerak, Ewelina Macech-Klicka, Maciej Wakula, Rafal Tomecki, Krzysztof Goryca, Malgorzata Rydzanicz, Mateusz Chmielarczyk, Malgorzata Szostakowska-Rodzos, Marta Wisniewska, Filip Lyczek, Aleksandra Helwak, David Tollervey, Grzegorz Kudla, Ewa A. Grzybowska
Format: | Article |
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Diterbitkan: | MDPI AG 2022-09-01 |
Deskripsi
HAX1 is a human protein with no known homologues or structural domains. Mutations in the <i>HAX1</i> gene cause severe congenital neutropenia through mechanisms that are poorly understood. Previous studies reported the RNA-binding capacity of HAX1, but the role of this binding in physiology and pathology remains unexplained. Here, we report the transcriptome-wide characterization of HAX1 RNA targets using RIP-seq and CRAC, indicating that HAX1 binds transcripts involved in translation, ribosome biogenesis, and rRNA processing. Using CRISPR knockouts, we find that HAX1 RNA targets partially overlap with transcripts downregulated in <i>HAX1</i> KO, implying a role in mRNA stabilization. Gene ontology analysis demonstrated that genes differentially expressed in <i>HAX1</i> KO (including genes involved in ribosome biogenesis and translation) are also enriched in a subset of genes whose expression correlates with <i>HAX1</i> expression in four analyzed neoplasms. The functional connection to ribosome biogenesis was also demonstrated by gradient sedimentation ribosome profiles, which revealed differences in the small subunit:monosome ratio in <i>HAX1</i> WT/KO. We speculate that changes in <i>HAX1</i> expression may be important for the etiology of HAX1-linked diseases through dysregulation of translation.