PEAC-seq adopts Prime Editor to detect CRISPR off-target and DNA translocation
oleh: Zhenxing Yu, Zhike Lu, Jingjing Li, Yingying Wang, Panfeng Wu, Yini Li, Yangfan Zhou, Bailun Li, Heng Zhang, Yingzheng Liu, Lijia Ma
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-12-01 |
Deskripsi
It is still a challenge to accurately identify off-target endonuclease edits. Here the authors report PEAC-seq using a Prime Editor to insert a tag to the editing sites and enrich the tagged regions with site-specific primers for sequencing: they show that PEAC-seq could identify DNA translocations.