Whole Genome Sequence of <i>Dermacoccus</i> <i>abyssi </i>MT1.1 Isolated from the Challenger Deep of the Mariana Trench Reveals Phenazine Biosynthesis Locus and Environmental Adaptation Factors
oleh: Wael M. Abdel-Mageed, Bertalan Juhasz, Burhan Lehri, Ali S. Alqahtani, Imen Nouioui, Dawrin Pech-Puch, Jioji N. Tabudravu, Michael Goodfellow, Jaime Rodríguez, Marcel Jaspars, Andrey V. Karlyshev
| Format: | Article |
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| Diterbitkan: | MDPI AG 2020-02-01 |
Deskripsi
<em>Dermacoccus abyssi </em>strain MT1.1<sup>T</sup> is a piezotolerant actinobacterium that was isolated from Mariana Trench sediment collected at a depth of 10898 m. The organism was found to produce ten dermacozines (A‒J) that belonged to a new phenazine family and which displayed various biological activities such as radical scavenging and cytotoxicity. Here, we report on the isolation and identification of a new dermacozine compound, dermacozine M, the chemical structure of which was determined using 1D and 2D-NMR, and high resolution MS. A whole genome sequence of the strain contained six secondary metabolite-biosynthetic gene clusters (BGCs), including one responsible for the biosynthesis of a family of phenazine compounds. A pathway leading to the biosynthesis of dermacozines is proposed. Bioinformatic analyses of key stress-related genes provide an insight into how the organism adapted to the environmental conditions that prevail in the deep-sea.