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The most important volatile in determining the aroma of fragrant rice is 2-Acetyl-1-pyrroline (2-AP); however, the transcriptional regulation mechanism of 2-AP biosynthesis in fragrant rice is still unclear. In this study, <i>Osp5cs1</i> knockout mutant lines and <i>OsP5CS1</i> over-expression lines were constructed by the genetic transformation of the Indica rice cultivar, i.e., ‘Zhonghua11′, which knocks out <i>OsBADH2</i> to produce fragrance in aromatic rice. The <i>OsP5CS1</i> gene was also identified as a key gene in the 2-AP biosynthesis pathway of aromatic rice. The <i>OsP5CS1</i> promoter was used as bait, and the OsbZIP60-like transcription factor was screened by yeast one-hybrid assays. The OsbZIP60-like transcription factor specifically bound to the <i>OsP5CS1</i> gene. The dual luciferase reporting system found that the OsbZIP60-like transcription factor promoted the transcriptional activation of <i>OsP5CS1</i>. Compared with the wild type, <i>OsP5CS1</i> gene expression was significantly down-regulated in the <i>Osbzip60</i>-like mutant and resulted in a substantial reduction in 2-AP biosynthesis. Moreover, the <i>OsP5CS1</i> gene expression was significantly up-regulated in <i>OsbZIP60</i>-like over-expressed plants, and the 2-AP concentrations were also increased, whereas the <i>Osbzip60</i>-like mutants were found to be sensitive to Zn deficiency. Overall, the OsbZIP60-like transcription factor promoted the 2-AP accumulation. This study provides a theoretical basis for the transcriptional regulation mechanism of 2-AP biosynthesis and explores the function of the OsbZIP transcription factor in fragrant rice.