Mapping RNA Sequences that Contact Viral Capsid Proteins in Virions

oleh: C. Cheng Kao, Ella Chuang, James Ford, Jie Huang, Ram Podicheti, Douglas Rusch

Format: Article
Diterbitkan: Bio-protocol LLC 2017-07-01

Deskripsi

We have adapted the methodology of CLIP-seq (Crosslinking-Immunoprecipitation and DNA Sequencing) to map the segments of encapsidated RNAs that contact the protein shells of virions. Results from the protocol report on the RNA sequences that contact the viral capsid.