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Probiotics are a potential strategy for salmonellosis control. A defined pig microbiota (DPM) mixture of nine bacterial strains previously exhibited probiotic and anti-<i>Salmonella</i> properties in vitro. Therefore, we evaluated its gut colonization ability and protection effect against <i>S. typhimurium</i> LT2-induced infection in the gnotobiotic piglet model. The DPM mixture successfully colonized the piglet gut and was stable and safe until the end of the experiment. The colon was inhabited by about 9 log CFU g⁻¹ with a significant representation of bifidobacteria and lactobacilli compared to ileal levels around 7–8 log CFU g⁻¹. Spore-forming clostridia and bacilli seemed to inhabit the environment only temporarily. The bacterial consortium contributed to the colonization of the gut at an entire length. The amplicon profile analysis supported the cultivation trend with a considerable representation of lactobacilli with bacilli in the ileum and bifidobacteria with clostridia in the colon. Although there was no significant <i>Salmonella</i>-positive elimination, it seems that the administered bacteria conferred the protection of infected piglets because of the slowed delayed infection manifestation without translocations of <i>Salmonella</i> cells to the blood circulation. Due to its colonization stability and potential protective anti-<i>Salmonella</i> traits, the DPM mixture has promising potential in pig production applications. However, advanced immunological tests are needed.