Protocol for controlled cortical impact in human cerebral organoids to model traumatic brain injury
oleh: Santiago Ramirez, Abhisek Mukherjee, Sofia E. Sepulveda, Camila Gherardelli, Andrea Becerra-Calixto, Nicolas Bravo-Vasquez, Claudio Soto
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-12-01 |
Deskripsi
Summary: Modeling traumatic brain injury (TBI) has been a challenge. Rodent and cellular models have provided relevant contributions despite their limitations. Here, we present a protocol for a TBI model based on the controlled cortical impact (CCI) performed on human cerebral organoids (COs), self-assembled 3D cultures that recapitulate features of the human brain. Here, we generate COs from iPSCs obtained from reprogrammed fibroblasts.For complete details on the use and execution of this protocol, please refer to Ramirez et al. (2021).