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Interspecific hybridization of rapeseed is an important way to innovate breeding resources. This research used <i>Brassica napus</i> and <i>Brassica rapa</i> for artificial synthesis interspecific hybridization of F₁. The F₁ self-fruiting rate was particularly low. By comparing the fertilization rate and seed setting rate of nine crosses and selfing combinations of interspecific hybrid progeny F₁ and control <i>B. napus</i>, the results proved that the genetic stability of egg cells was greater than that of sperm cells, so the F₁ could get seed by artificial pollination with other normal pollen. Based on these results, interspecific maternal inbred offspring (induced F₁) from egg cells was obtained by emasculation and pollination with the pollen of DH inducer Y3380. It was found through morphological analysis, flow cytometry identification, and meiotic observation of induced F₁, the plants had most normal fertile tetraploid and the meiosis was normal. The FISH results showed that the induced F₁ were <i>B. napus</i> (2n = 4x = 38, AACC), 20 A and 19 C chromosomes. The results of SNP chip detection and genetic cluster analysis found that the genetic variation between interspecies could be preserved or broadened in the induced F₁. The use of DH inducer created special breeding resources for interspecific hybridization and distant hybridization of rapeseed while shortening time, improving efficiency, and providing a new insight into innovate breeding resources.