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Three new substituted bithiophenes (<b>1</b>&#8315;<b>3</b>), and one new sulf-polyacetylene ester, ritroyne A (<b>16</b>) were isolated from the whole plant of <i>Echinops ritro</i> together with twelve known substituted thiophenes. The structures were elucidated on the basis of extensive spectroscopic analysis including 1D and 2D NMR as well as MS. Furthermore, the absolute configuration of ritroyne A (<b>16</b>) was established by computational methods. In bioscreening experiments, four compounds (<b>2</b>, <b>4</b>, <b>12</b>, <b>14</b>) showed similar antibacterial activity against <i>Staphylococcus aureus</i> ATCC 2592 with levofloxacin (8 &#181;g/mL). Five compounds (<b>2</b>, <b>4</b>, <b>9</b>, <b>12</b>, <b>14</b>) exhibited antibacterial activities against <i>Escherichia coli</i> ATCC 25922, with minimum inhibitory concentration (MIC) values of 32&#8315;64 &#181;g/mL. Three compounds (<b>2</b>, <b>4</b>, <b>12</b>) exhibited antifungal activities against <i>Candida albicans</i> ATCC 2002 with MIC values of 32&#8315;64 &#181;g/mL. However, compound <b>16</b> did not exhibit antimicrobial activities against three microorganisms.