Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism
oleh: Martin Pelosse, Cécile Cottet-Rousselle, Cécile M. Bidan, Aurélie Dupont, Kapil Gupta, Imre Berger, Uwe Schlattner
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-03-01 |
Deskripsi
AMP-activated protein kinase AMPK senses and regulates cellular energy state. Here the authors engineer a synthetic sensor, AMPfret, that allows direct, real-time readout of the AMPK conformational state by fluorescence resonance energy transfer (FRET).