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Evaluation of the Eazyplex<sup>®</sup> <i>Candida</i> ID LAMP Assay for the Rapid Diagnosis of Positive Blood Cultures
oleh: Arvid Berlau, Sylvia Stoll, Birgit Edel, Bettina Löffler, Jürgen Rödel
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2024-09-01 |
Deskripsi
Rapid molecular assays can be used to identify <i>Candida</i> pathogens directly from positive blood cultures (BCs) in a timely manner compared to standard methods using subcultures. In this study, the eazyplex<sup>®</sup> <i>Candida</i> ID assay, which is based on loop-mediated amplification (LAMP) and is currently for research use only, was evaluated for the identification of the most common fungal species. A total of 190 BCs were analysed. Sensitivity and specificity were 93.88% and 99.26% for <i>C. albicans</i>, 89.13% and 100% for <i>Nakaseomyces glabratus</i> (<i>N. glabratus</i>), 100% and 100% for <i>Pichia kudravzevii</i> (<i>P. kudriavzevii</i>), 100% and 100% for <i>C. tropicalis,</i> and 100% and 99.44% for <i>C. parapsilosis</i>. Sample preparation took approximately 11 min and positive amplification results were obtained between 8.5 and 19 min. The eazyplex<sup>®</sup> <i>Candida</i> ID LAMP assay is an easy-to-use diagnostic tool that can optimise the management of patients with candidemia.