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In addition to increasing the efficacy of various drugs, Nanoparticles reduce their side effects. In this study, different nanoparticle formulations of Doxorubicin anticancer drugs were prepared. The efficacy of the formulations produced in the cell culture medium was studied compared with the free drug. Reverse phase evaporation was used to form the liposome containing doxorubicin. The graphite nanoparticles were prepared. These nanoparticles were mixed with the liposome containing doxorubicin, and the related Nano-complex was conjugated. Spectroscopy methods for visible light-ultraviolet, light and light dynamics differentiation were used to describe nanoparticles. For the toxicity of different formulation, MTT and MCF-7 cells were used. The amount of drug loading in the liposomes was 72%. The largest amount was related to the Nano-conjugated complex and the smallest size was related to the graphene-oxide nanoparticle with a nanometer size. The controlled release in 96 hours and the amount of drug release was 95.43%. Doxorubicin-containing liposome toxicity was 75% and Nano- conjugated complex was 85%, at the lowest drug concentration (10µM). The free drug created 35% cell toxicity in 10µM and 89% in 2500µM. The results of the study showed Liposomes act as a suitable nanoparticle for doxorubicin. It was found that the effect of nanoparticles of graphene oxide is very important. In the presence of this nanoparticle in the complex, toxicity increased significantly.