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Abstract Satellite observations of anthropogenic carbon dioxide (CO2) emissions within urban settings offer unique potential to understand carbon sources and sinks and evaluate carbon mitigation strategies. Despite availability of column‐averaged dry air mole fraction of CO2 ( XCO2) from Orbiting Carbon Observatory‐2 (OCO‐2), temporal variations of XCO2 and their drivers in cities remain poorly understood due to inconsistent definitions of urban extent, diverse urban forms, and unresolved impacts of urban vegetation on carbon fluxes. To this end, this study revealed that OCO‐2 XCO2 measurements from 2014 to 2018 exhibited statistically significant seasonal and trend components for each city. A correlation analysis suggested a weak association between XCO2 trends and fossil fuel CO2 emissions ( FFCO2) trends but a close relationship between yearly average XCO2 and FFCO2 trends. Vegetation abundance exhibited a negative relationship with the XCO2 seasonality, though it only explained 21% of the variance. No statistically significant relationship between urban morphological factors (areal extent, complexity, and compactness) and temporal XCO2 components was observed. However, urban morphological factors had a close relationship with the total amount of FFCO2 aggregated over the study period. Thus, it was speculated that urban morphological factors exerted their influence on XCO2 through fossil fuel consumption. When only cities of high normalized difference vegetation index seasonality were used, statistically significant correlation coefficients between urban morphological factors and winter/summer averaged XCO2 measurements were found. The variations of these correlation coefficients between leaf‐on and leaf‐off seasons stress the important role that urban trees play in mitigating carbon emissions in cities.