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Activation of local translation in neurites in response to stimulation is an important step in the formation of long-term memory (LTM). CPEB proteins are a family of translation factors involved in LTM formation. The <i>Drosophila</i> CPEB protein Orb2 plays an important role in the development and function of the nervous system. Mutations of the coding region of the <i>orb2</i> gene have previously been shown to impair LTM formation. We found that a deletion of the 3’UTR of the <i>orb2</i> gene similarly results in loss of LTM in <i>Drosophila.</i> As a result of the deletion, the content of the Orb2 protein remained the same in the neuron soma, but significantly decreased in synapses. Using RNA immunoprecipitation followed by high-throughput sequencing, we detected more than 6000 potential Orb2 mRNA targets expressed in the <i>Drosophila</i> brain. Importantly, deletion of the 3′UTR of <i>orb2</i> mRNA also affected the localization of the Csp, Pyd, and Eya proteins, which are encoded by putative mRNA targets of Orb2. Therefore, the 3′UTR of the <i>orb2</i> mRNA is important for the proper localization of Orb2 and other proteins in synapses of neurons and the brain as a whole, providing a molecular basis for LTM formation.