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A lipid extract was obtained from eggs of the sailfin sandfish, <i>Arctoscopus japonicus</i>. Immunostimulatory effects of <i>A. japonicus</i> lipids incorporated with PEG6000 (AJ-PEG) on immunosuppressed mice treated with cyclophosphamide (CY) were investigated. AJ-PEG was administered orally to mice at different concentrations of 25 to 100 mg/kg body weight (BW). CY was injected to mice intraperitoneally at 80 mg/kg BW. Administration of AJ-PEG significantly increased the spleen index of CY-treated mice. AJ-PEG also stimulated the proliferation of splenic lymphocytes and natural killer (NK) activity. Immune-associated cytokines such as <i>IL-1β</i>, <i>IL-2</i>, <i>IL-4</i>, <i>IL-6</i>, <i>TNF-α</i>, and <i>IFN-γ</i> as well as <i>TLR4</i> were overexpressed in splenic lymphocytes. Furthermore, AJ-PEG significantly increased splenic CD4+ and CD8+ T lymphocytes. In peritoneal macrophages, AJ-PEG administration improved proliferation, nitric oxide (NO) production, and phagocytosis. It also upregulated <i>iNOS</i>, <i>COX-2</i>, <i>IL-1β</i>, <i>IL-6</i>, and <i>TNF-α</i> expression. Taken together, these results suggest that AJ-PEG can be used in animal models with immunosuppressive conditions as a potent immunomodulatory agent.