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The objective of this research was to evaluate the ruminal fermentation parameters and in vitro Greenhouse gas (GHG) production derived from the fermentation of a balanced sheep diet with the addition of vegetable oils (canola, corn, safflower, and sunflower) or glycerol at different proportions (0, 20, and 40 g/kg of dry matter, DM). The fermentations showed that the highest G_max was obtained with the addition of 40 g/kg of sunflower oil and 20 g/kg of glycerol with values of 180.97 and 179.95 mL/g DM, respectively. The treatment with 40 g/kg DM of corn oil showed the lowest values in CH₄ production (7.15 mL/g DM when compared to the control) and it seemed to be a potential feeding strategy for reducing GHG emissions without affecting gas production. However, the N-NH₃ content for this treatment in both doses (1.90 and 1.88 mg/dL) indicated that some toxicity for the animal could be expected.