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Four families with five species of acalyptrate Diptera are recorded for the first time from Sicily: Aulacigastridae [Aulacigaster falcata Papp, A. leucopeza (Meigen)], Odiniidae [Odinia trinotata Robineau-Desvoidy], Periscelididae [Periscelis annulata (Fallén)] and Carnidae [Meoneura freta Collin]. In six families that were previously known from Sicily, an additional nine new species records are given. The lists of species of Meoneura Rondani of Italy and of Herina Robineau-Desvoidy (Ulidiidae) of Sicily are updated.