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This study evaluated the usefulness and validity of wastewater SARS-CοV-2 monitoring in passenger ships. Wastewater grab samples (n = 54) were collected from seven cruise ships arriving at the port of Piraeus from June to November 2021. Samples were analyzed for the presence of SARS-CoV-2 RNA with an RT-PCR based method. Results were compared against the number of cases detected on the ships before arrival. It was demonstrated that sewage monitoring can detect the presence of SARS-CoV-2, even with few cases on board. Future efforts should focus on the collection of more representative samples to increase the consistency and validity of the investigated practice.