Novel CaF<sub>2</sub> Nanocomposites with Antibacterial Function and Fluoride and Calcium Ion Release to Inhibit Oral Biofilm and Protect Teeth

oleh: Heba Mitwalli, Abdulrahman A. Balhaddad, Rashed AlSahafi, Thomas W. Oates, Mary Anne S. Melo, Hockin H. K. Xu, Michael D. Weir

Format: Article
Diterbitkan: MDPI AG 2020-08-01

Deskripsi

(1) Background: The objective of this study was to develop a novel dental nanocomposite containing dimethylaminohexadecyl methacrylate (DMAHDM), 2-methacryloyloxyethyl phosphorylcholine (MPC), and nanoparticles of calcium fluoride (nCaF<sub>2</sub>) for preventing recurrent caries via antibacterial, protein repellent and fluoride releasing capabilities. (2) Methods: Composites were made by adding 3% MPC, 3% DMAHDM and 15% nCaF<sub>2</sub> into bisphenol A glycidyl dimethacrylate (Bis-GMA) and triethylene glycol dimethacrylate (TEGDMA) (denoted BT). Calcium and fluoride ion releases were evaluated. Biofilms of human saliva were assessed. (3) Results: nCaF<sub>2</sub>+DMAHDM+MPC composite had the lowest biofilm colony forming units (CFU) and the greatest ion release; however, its mechanical properties were lower than commercial control composite (<i>p</i> < 0.05). nCaF<sub>2</sub>+DMAHDM composite had similarly potent biofilm reduction, with mechanical properties matching commercial control composite (<i>p</i> > 0.05). Fluoride and calcium ion releases from nCaF<sub>2</sub>+DMAHDM were much more than commercial composite. Biofilm CFU on composite was reduced by 4 logs (<i>n</i> = 9, <i>p</i> < 0.05). Biofilm metabolic activity and lactic acid were also substantially reduced by nCaF<sub>2</sub>+DMAHDM, compared to commercial control composite (<i>p</i> < 0.05). (4) Conclusions: The novel nanocomposite nCaF<sub>2</sub>+DMAHDM achieved strong antibacterial and ion release capabilities, without compromising the mechanical properties. This bioactive nanocomposite is promising to reduce biofilm acid production, inhibit recurrent caries, and increase restoration longevity.