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This paper originally proposes a wireless multisensor module with illuminance, temperature, relative humidity (RH) and carbon dioxide (CO₂) sensors in an aseptic jar incubator for a solid-state fermentation (SSF) of <i>Cordyceps militaris</i> culture. The light intensity, ambient temperature, RH and CO₂ are the critical cultivation factors of <i>C. militaris</i>. First, these sensors are integrated in a multisensor platform which is installed inside a lid and covered with a high-efficiency particulate air (HEPA) membrane of class H14 for sterilization of bacteria and viruses. The observations of sensors are then transmitted by a wireless XBee network where the slave sensor node is fixed at the top of jar lid and the master radio node receives data and uploads to an on-site monitoring node. The acquired information is further transmitted to an iCloud database and displayed in a web-based monitoring system. The results illustrate the proposed wireless multisensor module was validated with sufficient accuracy, reliable confidence and well-tolerance for <i>C. militaris</i> cultivation biotechnology under aseptic conditions.