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Evaluation of health-related quality of life (HRQOL) in healthcare professionals (HP) is a crucial measure for improving their healthcare interventions and pharmaceutical care planning which they provide to their patients for prevention and better treatment of diseases. Studies have shown that poor health status among HPs is a greater risk factor in achieving desired outcomes in their patients. This study assessed the current level of HRQoL among HPs and evaluated various factors influencing their HRQoL. A cross-sectional study was conducted among HPs using the self-administered research tool “WHOQOL-BREF”. Descriptive, comparative, and inferential statistics were performed using SPSS ver. 24. The study results reported a total of 336 participants, with more males than females (n=268, 79.8%, and n=68, 20.2% respectively). The mean scores for the physical, psychological, social relationships and environmental domains were 65.18±13.01, 68.92±15.53, 70.30±15.90, and 65.10±15.17, respectively. This study confirmed that HPs in Pakistan were more satisfied with their HRQoL in social domain and relatively less satisfied in physical and environment domains of HRQoL.