Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

oleh: Xiao Fan, Jia Wang, Xing Zhang, Zi Yang, Jin-Can Zhang, Lingyun Zhao, Hai-Lin Peng, Jianlin Lei, Hong-Wei Wang

Format: Article
Diterbitkan: Nature Portfolio 2019-06-01

Deskripsi

It remains a challenge to obtain high-resolution structures of molecules smaller than 200 kDa using single particle cryo-EM. Here, the authors apply the Cs-corrector-VPP coupled cryo-EM and solve structures of the 52 kDa streptavidin (SA) protein at near-atomic resolution.