Find in Library

The complete mitochondrial genome of an orb-weaving spider Neoscona multiplicans was determined. The circular mitogenome is 14,074 bp in length (GenBank accession number MK052682), consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA genes, and an A + T-rich region. The gene arrangement and orientation of N. multiplicans are identical to those found in other known spiders. The nucleotide composition is significantly biased toward A + T content at 74.82% (A: 35.44%; C: 9.53%; G: 15.65%; T: 39.38%). ATT, ATA, TTA, TTG were initiation codons and TAA, TAG, T were termination codons. Six intergenic regions and 24 reading frame overlaps were found in the mitogenome of N. multiplicans. The A + T-rich is 483 bp with an A + T content of 77.23%. Eleven tRNAs lack the potential to form the cloverleaf-shaped secondary structure. Phylogenetic tree based on 13 PCGs showed that N. multiplicans is closely related to Araneus ventricosus, which is consistent with the conventional classification.