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The cuticle is the outermost layer of plant aerial tissue that interacts with the environment and protects plants against water loss and various biotic and abiotic stresses. ADP ribosylation factor guanine nucleotide exchange factor proteins (ARF-GEFs) are key components of the vesicle trafficking system. Our study discovers that AtMIN7, an <i>Arabidopsis</i> ARF-GEF, is critical for cuticle formation and related leaf surface defense against the bacterial pathogen <i>Pseudomonas syringae</i> pathovar tomato (<i>Pto</i>). Our transmission electron microscopy and scanning electron microscopy studies indicate that the <i>atmin7</i> mutant leaves have a thinner cuticular layer, defective stomata structure, and impaired cuticle ledge of stomata compared to the leaves of wild type plants. GC–MS analysis further revealed that the amount of cutin monomers was significantly reduced in <i>atmin7</i> mutant plants. Furthermore, the exogenous application of either of three plant hormones—salicylic acid, jasmonic acid, or abscisic acid—enhanced the cuticle formation in <i>atmin7</i> mutant leaves and the related defense responses to the bacterial <i>Pto</i> infection. Thus, transport of cutin-related components by AtMIN7 may contribute to its impact on cuticle formation and related defense function.