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The domains of unknown function (DUF) superfamilies contain proteins with conserved amino acid sequences without known functions. Among them, DUF668 was indicated widely involving the stress response of plants. However, understanding ZoDUF668 is still lacking. Here, 12 <i>ZoDUF668</i> genes were identified in ginger by the bioinformatics method and unevenly distributed on six chromosomes. Conserved domain analysis showed that members of the same subfamily had similar conserved motifs and gene structures. The promoter region of <i>ZoDUF668s</i> contained the light, plant hormone and stress-responsive elements. The prediction of miRNA targeting relationship showed that nine ginger miRNAs targeted four <i>ZoDUF668</i> genes through cleavage. The expression patterns of 12 <i>ZoDUF668</i> genes under biotic and abiotic stress were analyzed using RT-qPCR. The results showed that the expression of seven <i>ZoDUF668</i> genes was significantly downregulated under <i>Fusarium solani</i> infection, six <i>ZoDUF668</i> genes were upregulated under cold stress, and five <i>ZoDUF668</i> genes were upregulated under waterlogging stress. These results indicate that the <i>ZoDUF668</i> gene has different expression patterns under different stress conditions. This study provides excellent candidate genes and provides a reference for stress-resistance research in ginger.