D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor
oleh: Sabato D’Auria, Annalisa Vitale, Luigi Zeni, Tullio Labella, Pierangelo Orlando, Giuseppe Ruggiero, Maria Staiano, Micaela Tartaglia, Luisa Iozzino, Paola Ringhieri, Nunzio Cennamo, Vincenzo Aurilia, Viviana Scognamiglio
| Format: | Article |
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| Diterbitkan: | MDPI AG 2007-10-01 |
Deskripsi
D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care.