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The four nitrogen-containing heterocyclic compounds uridine (1), 2-(β-D-glucopyranosyl)-3-isoxazolin-5- one (2), adenosine (3), and hypaphorine (4) were isolated from the n-butanol extract of the roots of Callerya speciosa. Their structures were characterized on the basis of extensive nuclear magnetic resonane (NMR), mass spectroscopic analyses, and comparison with reported values. This is the first report on the isolation of compounds 1-4 from Callerya speciosa.